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An overview of the immunocolloidal-gold technology
Technology based upon immunocolloidalgold (often known as “colloidal”) is used as a tracer in the detection of antigen antibodies. The colloidal “colloidal” gold was introduced by Faulk equals 1971 immunochemical. In 1980, Leuvering published a method to determine the human chorionicgonadotropin (mCG). This technology has become very popular in areas such as medical clinical inspection, food security inspection, and animal epidemic surveillance. Coloidal gold immunofiltration (CGI) and colloidal Gold immunochromatography (CGI) are two of the most popular and widely used techniques within the veterinary industry.
Coloidal gold immunolabeling technology
The colloidal gold, which is positively charged when placed in weak bases environment, can form an electrical bond with negatively charged proteins molecules. It does not alter the biochemical properties of the proteins. Due to the high amount of surface charge, colloidal silver particles are strong at binding proteins. To make colloidal golden proteins, you can noncovalently combine coloidal gold particles with staphylococcus A (SPA), staphylococcus A (IgG), toxin, hormone, enzyme, antibiotic and bovine serumalbumin peptide conjugate. If the markers are made from gold particles of high electron densities, visible pink spots could be formed when they aggregate at an antigen body reaction spot on the solid-phase carrier. Therefore, colloidal gold, as a marker, can be used in immunoelectron microscopy, conventional light microscopy, quantitative and qualitative studies of antigens, as well as qualitative or semi-quantitative rapid detection of antigens or antibodies in vitro immunochromatography.
Benefits of the colloidal-gold immunolabeling technique
Immunocolloidal-gold technology is stable and easy to preserve. It is possible to preserve the results of experiments for long periods. This operation is quick and easy. The results can also be easily observed and compared. It is as simple as immersing the strips into the specimen. After the immunoanalysis, the colloidal gold will be used to indicate the process of forming the color mark. The entire detection procedure takes between 3-10 minutes and is non-toxic to the operator. There are no radioisotopes and o-phenylenediamines that can pollute the environment or cause injury to the operator in colloidal gold immunodetection. There is no harm in gold or silver, and there are no environmental pollutants. The commercial reagent doesn’t require any instruments, which saves money, makes it easier to use, and allows for more field applications.
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